Three of the deceased were identified as Mehedi Matubbar, 25, Hafizul Islam Habu, 40, and Sirajul Islam, 25.
The accident occurred on Dhaka-Khulna highway on Friday evening.
Bhanga Highway Police Station Officer in Charge Abu Sayeed Md Khairul Anam said, the accident took place when a Dhaka-bound bus of Sohag Paribahan collided with a leguna coming from the opposite direction.
Two of the injured, Rani Bala, 60, and Pabitra Bala, 35, were taken to Faridpur Bangabandhu Sheikh Mujib Medical College Hospital after their condition deteriorated.